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Chemical footprinting has been used for over five decades now to use solvent accessibility as a way to determine structural information on proteins and nucleic acids. The idea of using X-rays to for footprinting, however, is relatively new. X-ray footprinting was first conceived and tried out at the National Synchrotron Light Source at Brookhaven National Laboratory in the late 90's, by Drs. Mark Chance and Michael Brenowitz. Since then, the technique has grown in popularity and been used to study a wide range of protein and nucleic acid systems, from small proteins to large complexes to ribosome motion within cells. Drs Sayan Gupta and Corie Ralston have now introduced the technique at the Advanced Light Source and Lawrence Berkeley National Laboratory. Read more below to learn more about how the technique works, and how it is used.