Recent publications from footprinting experiments performed at the ALS 

"Integrated Structural Studies for Elucidating Carotenoid-Protein Interactions," C.Y. Ralston, C.A. Kerfeld, Adv Exp Med Biol, 2021 May 8. doi:10.1007/5584_2020_615. Online ahead of print 

"Structure of an affinity-matured inhibitory recombinant fab against urokinase plasminogen activator reveals basis of potency and specificity,"  N. Sevillano, M.F. Bohn, M Zimanyi, Y. Chen, C. Petzold, S. Gupta, C.Y. Ralston, C.S. Craik. BBA Proteins and Proteomics, V1869, Issue 2, February 2021, 

“An ultra-high affinity synthetic nanobody blocks SARS-CoV-2 infection by locking Spike into an inactive conformation,” M. Schoof, B. Faust, Reuben A. Saunders, S. Sangwan, V. Rezelj, N.Hoppe, M. Boone, C.B. Billesbølle, M. Zimanyi, I. Deshpande, J. Liang, A.A. Anand, N. Dobzinski, B.S. Zha, B. Barsi-Rhyne, V. Belyy, Andrew W. Barile-Hill, S. Gupta, C.R. Simoneau, K. Leon, K.M. White, S. Nock, Y. Liu, N.J. Krogan, C.Y. Ralston, D. L. Swaney, A. García-Sastre, M. Ott, M. Vignuzzi, P. Walter, A. Manglik. Science, 2020 Dec 18;370(6523):1473-1479. doi: 10.1126/science.abe3255. Epub 2020 Nov 5

"Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP," M.A. Dominguez-Martin, M. Hammel, S. Gupta, S. Lechno-Yossef, M. Sutter, D.J. Rosenberg, Y. Chen, C.J. Petzold, C.Y. Ralston, T. Polika, C. A. Kerfeld, Scientific Reports 2020, v10, Article number: 15564. DOI:10.1038/s41598-020-72383-y

"Allosteric Priming of E. coli CheY by the Flagellar Motor Protein FliM," P. Wheatley, S. Gupta, A. Pandini, Y. Chen, C.J. Petzold, C.Y. Ralston, D.F. Blair, and S. Khan, Biophysical Journal  2020, 119(6), 1108-1122 doi:10.1016/j.bpj.2020.08.009. Biosciences highlight and accompanying movie can be found here

"Fast protein footprinting by X-ray mediated radical trifluoromethylation," M Cheng, A. Asuri, J. Kiselar, G. Mathai, M.R. Chance, M.L. Gross, J. Am. Soc. Mass Spectrometry 2020, 31(5), 1019-1024. DOI:10.1021/jasms.0c00085

"Development of container free sample exposure for synchrotron X-ray footprinting," S. Gupta, Y. Chen, C.J. Petzold, D.P.  DePonte, C.Y. Ralston,  Analytical Chemistry  2020, 92, 1, 1565–1573. DOI:10.1021/acs.analchem.9b04849

"POT1-TPP1 differentially regulates telomerase via POT1 His266 and as a function of single-stranded telomere DNA length," M. Xu, J. Kiselar, T.L.Whited , W. Hernandez-Sanchez, D.J. Taylor, Proceedings National Academy of Sciences 2019,  Nov 19, 116(47):23527-23533.  Epub 2019 Nov 4. DOI: 10.1073/pnas.1905381116

"Comparative ultrafast spectroscopy and structural analysis of OCP1 and OCP2 from Tolypothrix," V. Kuznetsova, M.A. Dominguez-Martin, H. Bao, S. Gupta, M. Sutter, M. Kloz, M. Rebarz, M. Precek, Y. Chen, C.J. Petzold, C.Y. Ralston, C.A Kerfeld, T. Polivka,  Biochimica et Biophysica  Acta Bioenergetics, online Nov 14 2019. DOI:10.1016/j.bbabio.2019.148120

"X-ray radiolytic labeling reveals the molecular basis of orange carotenoid protein photoprotection and its interactions with fluorescence recovery protein," S. Gupta, M. Sutter,  S.G. Ramesh, M.-A. Dominguez-Martin, H. Bao, X.A. Feng, L.J.G. Chan, C.J. Petzold, C.A. Kerfeld, C.Y. Ralston,  J. Biol. Chem. 2019, DOI: 10.1074/JBC.RA119.007592

"Heterohexamers Formed by CcmK3 and CcmK4 Increase the Complexity of Beta Carboxysome Shells," M. Sommer, M. Sutter, S. Gupta, H. Kirst, A. Turmo, S. Lechno-Yossef, R.L. Burton, C. Saechao, N.B. Sloan, X. Cheng, L.-J. Chan, C.J. Petzold, M. Fuentes-Cabrera, C.Y. Ralston, and C.A. Kerfeld, Plant Physiology 2019 179(1), 156-167. DOI:

"Recent Advances in X-Ray Hydroxyl Radical Footprinting at the Advanced Light Source Synchrotron," S.A. Morton, S. Gupta, C.J. Petzold, C.Y. Ralston,  Protein and Peptide Letters, 2019 Volume 26, Number 1, pp. 70-75(6), DOI:10.2174/0929866526666181128125725

"Water molecules mediate zinc mobility in the bacterial zinc diffusion channel ZIPB," S. Gupta, C. Merriman, C.J.  Petzold, C.Y. Ralston, D. Fu, "  J. Biol. Chem. 2019 Sep 6;294(36):13327-13335. doi: 10.1074/jbc.RA119.009239. Epub 2019 Jul 18. PubMed PMID: 31320477; PubMed Central PMCID: PMC6737223.

"Using X-ray Footprinting and Mass Spectrometry to Study the Structure and Function of Membrane Proteins," S. Gupta, Protein and Peptide Letters, Volume 26, Number 1, 2019, pp. 44-54(11)  DOI:10.2174/0929866526666181128142401

"A Metastable Contact and Structural Disorder in the Estrogen Receptor Transactivation Domain," Yi Peng, Shufe Cao, Janna Kiselar, Xiangzhu Xiao, Zhanwen Du, An Hsieh, Soobin Ko, Yinghua Chen, Prashansa Agrawal, Wenwei Zheng, Wuxian Shi, Wei Jiang, Lin Yang, Mark R. Chance, Witold K. Surewicz, Matthias Buck, Sichun Yang, Structure 2019 Volume 27, Issue 2, 5 , Pages 229-240.e4,  DOI:10.1016/j.str.2018.10.026

 "Multidomain architecture of estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains," Huang, W., Peng, Y., Kiselar, J., Zhao, X., Albaqami, A., Mendez, D., Chen, Y.-H., Chakravarthy, S., Gupta, S., Ralston, C.Y., Kao, H.-Y., Chance, M., and Yang, S. Nature Communications, 2018, 9, Article number: 3520. DOI:10.1038/s41467-018-06034-2

"Diabetes reversal by inhibition of the low-molecular-weight tyrosine phosphatase" Nat. Chem. Biol. 2017 Jun;13(6):624-632. doi: 10.1038/nchembio.2344. Epub 2017 Mar 27.

"Activation of tumor suppressor protein PP2A inhibits KRAS-driven tumor growth," J. Clin. Invest. 2017 Jun 1;127(6):2081-2090. doi: 10.1172/JCI89548. Epub 2017 May 15.

“The Molecular Basis for Binding of an Electron Transfer Protein to a Metal Oxide Surface,” T. Fukushima, S. Gupta, B. Rad, J.A. Cornejo, C.J. Petzold, L.G. Chan, R.A. Mizrahi, C.Y. Ralston, and C.M. Ajo-Franklin, J. Am. Chem. Soc. 2017, 139 (36), pp 12647–12654.

"Advancements and Application of Microsecond X-ray Footprinting at the Advanced Light Source," S. Gupta, R. Celestre, J. Feng, C.Y. Ralston, Synchrotron Radiation News 2016 V29 No1, p39.

“Recent Advances and Applications in Synchrotron X-ray Protein Footprinting for Protein Structure and Dynamics Elucidation," S. Gupta, J. Feng, M.R. Chance, C.Y. Ralston,  Protein & Peptide Letters 2016 Vol. 23, No. 3.

“Probing the Structure of Ribosome Assembly Intermediates In vivo using DMS and Hydroxyl Radical Footprinting,” R. Hulscher, J. Bohon, M. Rappe, S. Gupta, R. D’Mello, M. Sullivan, C.Y. Ralston, M.R. Chance, S.A. Woodson,  Methods 2016 Mar 22, S1046-2023(16) 30046-9.

“Synchrotron X-ray Footprinting as a Method to Visualize Water in Proteins,”  S. Gupta, J. Feng, C.J. Petzold, C.Y. Ralston, J. Synchrotron Radiation, 2016 Sep 1;23(Pt 5):1056-69. doi: 10.1107/S1600577516009024. Epub 2016 Jul 27.

 "Carotenoid translocation in the Orange Carotenoid Protein activates a photoprotective mechanism in cyanobacteria,"  R.L. Leverenz, M. Sutter, A. Wilson, S. Gupta, A. Thurotte, C. Boucier de Carbon, C. Petzold, C. Ralston, D. Kirilovsky, C.A. Kerfeld, Science 2015 V348, 6242, p1463.

"Local and global structural drivers for the photoactivation of the orange carotenoid protein," S. Gupta, M. Guttman, R.L. Leverenz, K. Zhumadilova, E.G. Pawlowski, C.J. Petzold, K.K. Lee, C. Ralston, C.A. Kerfeld,  Proceedings National Academy of Sciences 2015 V112 No41, E5567.

 "Oxidative footprinting in the study of structure and function of membrane proteins: current state and perspectives," V.N. Bavro, S. Gupta, C. Ralston, Biochemical Society Transactions, 2015, V43(5), p983

"Synchrotron footprinting on tour," J. Bohon, R. D'Mello, C. Ralston, S. Gupta, M. R Chance,  J. Sync. Rad., 2014 21, 24-31.

"Development of a microsecond x-ray protein footprinting facility at the Advanced Light Source," S. Gupta, R. Celestre, C. Petzold, M.R. Chance, C. Ralston,  J. Sync. Rad., 2014 21, 690.